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Investigating the effect of oil spills
on the environment and public health.
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Demetri D. Spyropoulos

Medical University of South Carolina

Marine Biomedicine and Environmental Sciences

HCC Room H0354
86 Jonathan Lucas St.,
Charleston , SC  29425  

spyropdd@musc.edu

Project List:

Using Embryonic Stem Cell Fate to Determine Potential Adverse Effects of Petroleum/Dispersant Exposure

Year 3-5 Investigator Grants (RFP-II)Role: Principal Investigator, Project Data Point of Contact, Outreach Team

Publications & Presentations:

Journal Articles - 5

2016

Bowers, R. R., Temkin, A. M., Guillette, L. J., Baatz, J. E., & Spyropoulos, D. D. (2016). The commonly used nonionic surfactant Span 80 has RXRα transactivation activity, which likely increases the obesogenic potential of oil dispersants and food emulsifiers. General and Comparative Endocrinology, 238, 61–68. doi
Temkin, A. M., Bowers, R. R., Magaletta, M. E., Holshouser, S., Maggi, A., Ciana, P., et al. (2016). Effects of Crude Oil/Dispersant Mixture and Dispersant Components on PPARy Activity in Vitro and in Vivo: Identification of Dioctyl Sodium Sulfosuccinate (DOSS; CAS #577-11-7) as a Probable Obesogen. Ehp, 124(1). doi
Toyota, K., McNabb, N. A., Spyropoulos, D. D., Iguchi, T., & Kohno, S. (2016). Toxic effects of chemical dispersant Corexit 9500 on water fleaDaphnia magna: Toxic effects of corexit 9500 onDAPHNIA MAGNA. J. Appl. Toxicol., 37(2), 201–206. doi

2014

Baatz, J. E., Newton, D. A., Riemer, E. C., Denlinger, C. E., Jones, E. E., Drake, R. R., et al. (2014). Cryopreservation of Viable Human Lung Tissue for Versatile Post-thaw Analyses and Culture. In Vivo, 28(4), 411–423. url
Temkin, A. M., & Spyropoulos, D. D. (2014). Induced pluripotent stem cell technology and aquatic animal species. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 163(1), 3–13. doi

Conference Presentations - 52

2016

Brumbaugh, A., Williams, C., Guillette, L. J., Spyropoulos, D. D., & Kohno, S. (2016). Obesogenic activity of oil dispersant corexit 9500 in the American alligator. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16. openurl
Cantu, T., Bowden, J. A., Temkin, A., Spyropoulos, D. D., Guillette, L. J., & Jr. (2016). Eicosanomics: Novel Approaches to Investigate the Effect of Oil/Dispersant Exposure on Eicosanoid Biosynthesis in Sentinel Species. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16: Poster. openurl
Cantu, T., Bowden, J. A., Temkin, A., Spyropoulos, D. D., Guillette, L. J., & Jr. (2016). Life Lessons From Wildlife And Lou: The Tale Of Pansteatitis And Use Of Novel Eicosanomic Techniques To Assess Inflammation In Sentinel Species. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16. openurl
McNabb, N. A., Miyagawa, S., Iguchi, T., Spyropoulos, D. D., Guillette, L. J., & Kohno, S. (2016). Investigating estrogenicity of oil dispersant COREXIT® EC9500A in the American alligator and diamondback terrapin. In Annual Meeting of Society for Maternal Fetal Medicine. Portland, OR : 01/03/16-01/07/16: Poster. openurl
McNabb, N. A., Spyropoulos, D. D., Guillette, L. J., & Kohno, S. (2016). Investigating estrogenicity and developmental effects of the dispersant COREXIT® EC9500A in the American alligator. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16. openurl
McNabb, N. A., Spyropoulos, D. D., Guillette, L. J., & Kohno, S. (2016). Investigating Estrogenicity Of COREXIT In The American Alligator: Learning In The Field And Lab From Lou Guillette. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16. openurl
McNabb, N. A., Williams, C. E., Spyropoulos, D. D., Guillette, L. J., & Kohno, S. (2016). Investigating effects of COREXIT and CWAF on sex determination in the American alligator. In Palmetto Alligator Research and Management Symposium.. Georgetown, SC : 03/11/16. openurl
Spyropoulos, D. D. (2016). Introduction & Concluding Remarks on the Life, the Work and the Impact of Lou Guillette, Jr. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16. openurl
Spyropoulos, D. D., Temkin, A. M., Cantu, T. M., McNabb, N. A., Williams, C. E., Bowers, R. R., et al. (2016). From Oiled Stem Cells to Pregnant Women: Methodically Navigating Between Complex Environments and Human Health. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16: Poster. openurl
Temkin, A. M., Bowers, R. R., Baatz, J. E., & Spyropoulos, D. D. (2016). Characterizing DOSS as an Obesogen In Vivo. In Gordon Research Conference, Environmental Endocrine Disruptors. Sunday River, Newry, ME : 06/1916-06/24/16: Poster. openurl
Temkin, A. M., Bowers, R. R., Baatz, J. E., & Spyropoulos, D. D. (2016). Rapid and sensitive assay screening reveals multiple potential hormone disrupting activities in MC252 crude oil and different dispersants. In 25 Years of Endocrine Disruption Research: Past Lessons and Future Directions. Bethesda, MD : 9/18/16-9/20/16. openurl
Temkin, A. M., Bowers, R. R., Baatz, J. E., & Spyropoulos, D. D. (2016). Characterization of DOSS as on Obesogen In Vivo. In 7th Society of Environmental Toxicology and Chemistry (SETAC) World Congress/SETAC North America 37th Annual Meeting. Orlando, FL : 11/06/16-11/10/16: Poster. openurl
Temkin, A. M., Bowers, R. R., Guillette, L. J., Bowden, J. A., Kucklick, J. R., Baatz, J. E., et al. (2016). In vivo Exposure and Stem Cell Transcriptomics To Determine If Dioctyl Sodium Sulfosuccinate (DOSS) Is A Bona Fide Obesogen. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16: Poster. openurl
Temkin, A. M., Bowers, R. R., Magaletta, M. E., Holshouser, S., Maggi, A., Ciana, P., et al. (2016). DOSS in Corexit and in Laxatives: Finding a Poster Child for Encouraging Movement in Dispersant and Obesity Sciences. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16. openurl
Temkin, A. M., Bowers, R. R., Sojka, C., Anderson, M., Guillette, L. J., Bowden, J. A., et al. (2016). Rapid and sensitive assay screening reveals multiple potential hormone disrupting activities in MC252 crude oil and different dispersants. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16: Poster. openurl
Williams, C. E., Guillette, L. J., Spyropoulos, D. D., & Kohno, S. (2016). Estrogenic effects of CWAF exposure on sex determination in the American alligator. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16. openurl
Williams, C. E., Guillette, L. J., Spyropoulos, D. D., & Kohno, S. (2016). Effects of exogenous estradiol on gonadal sex determination in the American alligator during in vitro culture of the isolated gonad-adrenal-mesonephric complex. In Annual Meeting of the Society for Integrative and Comparative Biology. Portland, OR : 01/03/16-01/07/16: Poster. openurl
Williams, C. E., Spyropoulos, D. D., Guillette, L. J., & Kohno, S. (2016). Lessons in research and beyond from Lou Guillette – assessing the impacts of CWAF on sex determination in the American alligator. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2016. Tampa, FL : 02/01/16-02/04/16. openurl

2015

Bernhard, M. C., Spyropoulos, D. D., Guillette, L. J., & Kohno, S. (2015). Can tributyltin be used as a positive control for obesogenic exposure in the American alligator (Alligator mississippiensis)? In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2015. Houston, TX : 02/16/15-02/19/15: Poster. openurl
Brumbaugh, A., Spyropoulos, D. D., & Kohno, S. (2015). Obesogenic Activity of Oil Dispersant Components in the American Alligator Following the Deepwater Horizon Oil Spill. In 2015 Medical University of South Carolina, HML Poster Session of the Summer Undergraduate Research Program: Endocrine Disrupting Compounds, the Environment and Human Health.. Charleston, SC : 7/29/2015: Poster. openurl
Brumbaugh, A., Spyropoulos, D. D., & Kohno, S. (2015). Obesogenic Activity of Oil Dispersant Components in the American Alligator Following the Deepwater Horizon Oil Spill. In 2015 Medical University of South Carolina Summer Undergraduate Research Program Oral Presentations. Charleston, SC : 7/30/2015. openurl
Kohno, S., McNabb, N. A., Williams, C. E., Spyropoulos, D. D., Guillette, L. J., & Parrott, B. B. (2015). Long-term influences of environmental factors on sex determination and differentiation in the American alligator. In 63rd NIBB Conference: Environment to Bioresponse. Okazaki, Japan : 11/30/15-12/02/15. openurl
McNabb, N., Spyropoulos, D. D., Guillette, L. J., & Kohno, S. (2015). Effects Of COREXIT 9500 As A Potential Endocrine Disruptor On Sex Determination Of The American Alligator. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2015. Houston, TX : 02/16/15-02/19/15: Poster. openurl
McNabb, N. A., Guillette, L. J., Spyropoulos, D. D., & Kohno, S. (2015). Investigating estrogenicity and developmental effects of the dispersant COREXIT in the American alligator and diamondback terrapin. In 19th Annual College of Charleston Graduate Program in Marine Biology Student Research Colloquium. Charleston, SC : 11/14/15-11/14/15. openurl
McNabb, N. A., Miyagawa, S., Iguchi, T., Spyropoulos, D. D., Guillette, L. J., & Kohno, S. (2015). Investigating estrogenicity and developmental effects of the dispersant COREXIT in the American alligator and diamondback terrapin. In 63rd NIBB Conference: Environment to Bioresponse. Okazaki, Japan : 11/30/15-12/02/15: Poster. openurl
Sojka, C., Temkin, A. M., Bowers, R. R., & Spyropoulos, D. D. (2015). Effects of Crude Oil and Dispersant Mixtures on PGR and AR Activity. In 2015 Medical University of South Carolina, HML Poster Session of the Summer Undergraduate Research Program: Endocrine Disrupting Compounds, the Environment and Human Health.. Charleston, SC : 7/29/2015: Poster. openurl
Sojka, C., Temkin, A. M., Bowers, R. R., & Spyropoulos, D. D. (2015). Effects of Crude Oil and Dispersant Mixtures on PGR and AR Activity. In 2015 Medical University of South Carolina Summer Undergraduate Research Program Oral Presentations. Charleston, SC : 7/30/2015. openurl
Spyropoulos, D. D. (2015). Fetal Origins of Obesity: Metabolites & Biomarkers in Offspring from Pregnancy-Linked Exposure to the COREXIT Component DOSS. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2015. Houston, TX : 02/16/15-02/19/15. openurl
Temkin, A., Bowers, R., Baatz, J., Guillette, L., & Spyropoulos, D. (2015). Evaluating the endocrine disrupting potential of crude oil and dispersant via transactivation assay screens. In SETAC North America 36th Annual Meeting. Salt Lake City, UT : 11/01/15-11/05/15. openurl
Temkin, A., Bowers, R., Baatz, J., Guillette, L., & Spyropoulos, D. (2015). Identification and characterization of putative obesogens in crude oil and dispersants used in the deepwater horizon oil spill. In SETAC North America 36th Annual Meeting. Salt Lake City, UT : 11/01/15-11/05/15: Poster. openurl
Temkin, A., Bowers, R., Baatz, J., Guillette, L. J., Kohno, S., & Spyropoulos, D. D. (2015). Keynote Speaker: Empowering Awareness of Environmental Impacts on Women's Health – From Wildlife and Stem Cells to the Clinic and Personal Choices. In Carolina Womenâ??s Health Research Forum. Columbia, SC : 10/23/15-10/23/15. openurl
Temkin, A. M., Bowers, R. R., Guillette, L. J., & Spyropoulos, D. D. (2015). RFP-II: Using Embryonic Stem Cell Fate to Determine Potential Adverse Effects of Petroleum/Dispersant Exposure. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2015. Houston, TX : 02/16/15-02/19/15: Poster. openurl
Temkin, A. M., Bowers, R. R., Magaletta, M. E., Holshouser, S., Maggi, A., Ciana, P., et al. (2015). Predicting Long Term Impacts of Oil/dispersant Exposures on Human Health and Higher Trophic Organisms: Obesogenicity. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2015. Houston, TX : 02/16/15-02/19/15: Poster. openurl
Williams, C. E., Guillette, L. J., Spyropoulos, D. D., & Kohno, S. (2015). Estrogenic Effects Of CWAF Exposure In Vitro On Sex Determination Of The American Alligator. In 19th Annual College of Charleston Graduate Program in Marine Biology Student Research Colloquium. Charleston, SC : 10/02/15-10/02/15: Poster. openurl

2014

Andersen, M., Temkin, A. M., Bowers, R., & Spyropoulos, D. D. (2014). Assessing Crude Oil and Dispersant Activation of Nuclear Receptors. In MUSC Marine Biomedicine & Environmental Sciences Program, Summer Undergraduate Research Program Student Colloquium. James Island, SC : 07/29/14. openurl
Andersen, M., Temkin, A. M., Bowers, R., & Spyropoulos, D. D. (2014). Assessing Crude Oil and Dispersant Activation of Nuclear Receptors. In Summer Undergraduate Research Program, Final Presentation Day. Charleston SC : 07/30/14. openurl
Barrett, N., Temkin, A., Ellisor, D., Kohno, S., Guillette, L. J.,, & Spyropoulos, D. D. (2014). Histological Analysis of Adipose Tissue in the Tail of American Aliigators Exposed to Tributyltin in ovo. In MUSC Marine Biomedicine & Environmental Sciences Program, Summer Undergraduate Research Program Student Colloquium. James Island, SC : 07/29/14. openurl
Barrett, N., Temkin, A., Kohno, S., Guillette, L. J.,, & Spyropoulos, D. D. (2014). Histological Analysis of Adipose Tissue in the Tail of American Aliigators Exposed to Tributyltin in ovo. In Summer Undergraduate Research Program, Final Presentation Day. Charleston SC : 07/30/14. openurl
Bernhard, M., Kohno, S., Guillette, L. J.,, & Spyropoulos, D. D. (2014). Effects Of Ppar?/Rxr? Signaling Via In Ovo Tributyltin Exposure On Development Of The American Alligator, Alligator Mississippiensis. In College of Charleston Marine Biology Graduate Program Student Research Colloquium 2014. James Island, SC : 09/19/14. openurl
Bernhard, M. C., Spyropoulos, D. D., Guillette L. J., & Kohno, S. (2014). Does in ovo exposure to a known endocrine disruptor, tributyltin (TBT), skew sex ratios in the American alligator (Alligator mississippiensis)-. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2014. Mobile, AL: 01/26/14-01/30/14: Poster. openurl
Magaletta, M., Temkin, A. M., Bowers, R., & Spyropoulos, D. D. (2014). Sub-fraction Toxicities of Gulf Oil and Corexit Water Accommodated Fraction and Their Ability to Drive Stem Cell De-Differentiation at Sub-Lethal Exposure. In MUSC Marine Biomedicine & Environmental Sciences Program, Summer Undergraduate Research Program Student Colloquium. James Island, SC : 07/29/14. openurl
Magaletta, M., Temkin, A. M., Bowers, R., & Spyropoulos, D. D. (2014). Sub-fraction Toxicities of Gulf Oil and Corexit Water Accommodated Fraction and Their Ability to Drive Stem Cell De-Differentiation at Sub-Lethal Exposure. In Summer Undergraduate Research Program, Final Presentation Day. Charleston SC : 07/30/14. openurl
McCoy, J., Parrott, B., Guillette, L. J.,, & Spyropoulos, D. D. (2014). Do warmer eggs yield hyper-masculinized alligator hatchlings? In MUSC Marine Biomedicine & Environmental Sciences Program, Annual Student Research Open House. James Island, SC : 07/18/14: Poster. openurl
Patton, M. P., Temkin, A., Ellisor, D., Kohno, S., Guillette, L. J.,, & Spyropoulos, D. D. (2014). Using Molecular Markers to Rapidly Characterize Adipogenic Stem Cell Differentiation. In 2014 Annual Meeting of SICB. Austin TX: Poster. openurl
Spyropoulos, D. D. (2014). A Not-So-Exhaustive Look At the Ins & Outs And Ups & Downs of ESC/iPSC Manipulations. In 2014 Annual Meeting of the 2. Society for In Vitro Biologists and Cryobiologists. Savannah, GA : 06/01/2014-06/05/2014. openurl
Spyropoulos, D. D. (2014). Isolation and growth of embryonic and induced pluripotent stem cells. In 2014 Annual Meeting of the 2. Society for In Vitro Biologists and Cryobiologists. Savannah, GA : 06/01/2014-06/05/2014. openurl
Temkin, A. M., Bowers, R., Ellisor, D. L., Baatz, J. E., Kucklick, J., Kohno, S., et al. (2014). Predicting Long Term Impacts of MC252 Oil/COREXIT Exposures on Human Health and Higher Trophic Organisms: Obesogenicity. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2014. Mobile, AL: 01/26/14-01/30/14: Poster. openurl
Temkin, A. M., Bowers, R., & Spyropoulos, D. D. (2014). Predicting Long Term Impacts of Oil/dispersant Exposures on Human Health and Higher Trophic Organisms: Obesogenicity. In MUSC Marine Biomedicine & Environmental Sciences Program, Annual Student Research Open House. James Island, SC : 07/18/14: Poster. openurl

2013

Allen, E. M., Temkin, A. M., Newton D.A., Mancia, A., Baatz, J. E., & Spyropoulos, D. D. (2013). Pygmy Sperm Whale (Kogia breviceps) progenitors undergo adipogenic differentiation under low oxygen tension unlike terrestrial mammalian progenitors. In 6th Aquatic Animal Models for Human Disease. Milwaukee, WI, June 30, 2013: Poster. openurl
Ellisor, D. L., Cloy-McCoy, J. A., Cantu, T.M., Kohno, S., Baatz, J. E., Guillette, L. J., & Spyropoulos, D. D. (2013). Using Embryonic Stem Cell Fate to Determine Potential Adverse Effects of Petroleum/Dispersant Exposure. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2013. New Orleans, LA: 01/21/2013-01/23/2013: Poster. url
Ellisor, D. L., Cloy-McCoy, J. A., Cantu, T.M., Kohno, S., Baatz, J. E., Guillette, L. J., & Spyropoulos, D. D. (2013). Using Embryonic Stem Cell Fate to Determine Potential Adverse Effects of Petroleum/Dispersant Exposure. In Gulf of Mexico Oil Spill & Ecosystem Science Conference 2013. New Orleans, LA: 01/21/2013-01/23/2013. openurl
Temkin, A. M., Kucklick, J., & Spyropoulos, D. D. (2013). Predicting Long Term Impacts of Oil/dispersant Exposures on Human Health and Higher Trophic Organisms. In SETAC North America 34th Annual meeting. Nashville, TN , 11/17/14-11/21/14: Poster. openurl
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